REGULATION OF HUMAN CALCITONIN GENE-TRANSCRIPTION BY CYCLIC-AMP

被引：30

作者：

DEBUSTROS, A

BALL, DW

PETERS, R

COMPTON, D

NELKIN, BD

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,BALTIMORE,MD 21231

[2] JOHNS HOPKINS UNIV,SCH MED,CTR ONCOL,BALTIMORE,MD 21231

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92325-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcription of the human calcitonin (CT) gene is markedly increased by cAMP in the TT line of medullary thyroid carcinoma. This response is conferred by 5′ flanking DNA sequences located between -132 and -252 relative to the transcription initiation site. Within this region are an upstream cyclic AMP response element (CRE), a downstream CRE flanked by two octamer motifs, and two adjacent C-rich AP2-like sequences. In transfection experiments in TT cells, the downstream CRE, combined with CT promoter sequences, generated 70% of the maximal cAMP response. The upstream CRE and the C-rich elements conferred 10 and 30% of this response, respectively. In gel mobility shift assays, specific TT cell proteins bound to each of these sequences. Therefore, the cAMP response of the CT gene is complex, requiring multiple elements acting in concert. © 1992.

引用

页码：1157 / 1164

页数：8

共 27 条

[1] AMPLIFICATION OF THE TRANSCRIPTIONAL SIGNAL MEDIATED BY THE TANDEM CAMP RESPONSE ELEMENTS OF THE GLYCOPROTEIN HORMONE ALPHA-SUBUNIT GENE OCCURS THROUGH SEVERAL DISTINCT MECHANISMS [J].