INTRACELLULAR PROCESSING AND TRANSPORT OF JUNIN VIRUS GLYCOPROTEINS INFLUENCES VIRION INFECTIVITY

被引:18
作者
DAMONTE, EB
MERSICH, SE
CANDURRA, NA
机构
[1] Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, Ciudad Universitaria, Pabellon 2
关键词
JUNIN VIRUS; ARENAVIRUS; GLYCOPROTEIN PROCESSING; GLYCOPROTEIN TRANSPORT; MONENSIN;
D O I
10.1016/0168-1702(94)90131-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The influence of glycoprotein processing, cleavage and transport on Junin virus (JV) infectivity was investigated using monensin combined with lectin binding assays. Yields of extracellular virus were more significantly reduced than cell-associated virus, indicating that monensin inhibited the transport of infectious virus to the extracellular space on a late stage of the replicative cycle. Shown by lectin reactivity and immunoprecipitation, the intracellular processing of JV glycoproteins involved first the maturation of GPC oligosaccharides to a complex form and then the precursor cleavage which might occur late in transit through or exit from the Golgi cisternae. Cleavage of GPC to yield the mature GP38 as well as cell surface immunofluorescence were blocked by monensin. Thus, GP38 production together with glycoprotein transport to the cell membrane seemed to be required for the release of infectious virus from JV-infected cells.
引用
收藏
页码:317 / 326
页数:10
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