PROTEIN-KINASE REGULATES TUMOR-NECROSIS-FACTOR MESSENGER-RNA STABILITY IN VIRUS-STIMULATED ASTROCYTES

Infection of astrocytes with Newcastle disease virus stimulated the production of 1,2-diacylglycerol, and resulted in the kinase-dependent expression of mRNAs encoding tumor necrosis factor (TNF), interferon α and β, and interleukin 6. The half-life of TNF mRNA was significantly decreased in the presence of protein kinase inhibitors H-7 and staurosporine, but not in the presence of HA1004. In contrast to the decay of TNF mRNA, the half-lives of other cytokine mp NAs were only minimally affected by the kinase inhibitors. These data indicated that the stability of TNF mRNA was regulated through a novel, kinase-dependent pathway. © 1990, Rockefeller University Press., All rights reserved.