KINETICS OF PROTEASE HYDROLYSIS OF EXTENDED PEPTIDE-SUBSTRATES - MEASUREMENT BY FLOW-INJECTION ANALYSIS

被引：3

作者：

CHONG, GL ^{[1
]}

DAVIS, LC ^{[1
]}

REECK, GR ^{[1
]}

机构：

[1] KANSAS STATE UNIV AGR & APPL SCI,DEPT BIOCHEM,MANHATTAN,KS 66506

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 186卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90078-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of α-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates. © 1990.

引用

页码：269 / 272

页数：4

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