EVIDENCE FROM 2-PROTONIC-STATE REACTIVITY PROBE KINETICS THAT CHYMOPAPAIN IN FRESH NONFRUIT LATEX OF CARICA-PAPAYA CONSISTS OF MULTIPLE FORMS OF CHYMOPAPAIN-A - THE VALUE OF CATALYTIC SITE CHARACTERISTICS IN THE IDENTIFICATION, CLASSIFICATION, AND CHARACTERIZATION OF THE PAPAYA CYSTEINE PROTEINASES PAPAIN, THE CHYMOPAPAINS, AND PAPAYA PROTEINASE-OMEGAC

被引：10

作者：

BROCKLEHURST, K

MCKEE, R

SALIH, E

SMITH, H

机构：

[1] UNIV LONDON ST BARTHOLOMEWS HOSP,COLL MED,DEPT BIOCHEM,LONDON EC1M 6BQ,ENGLAND

[2] UNIV LEICESTER,DEPT BOT,LEICESTER LE1 7RH,ENGLAND

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1985年 / 4卷 / 02期

关键词：

D O I：

10.1007/BF01025371

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：103 / 127

页数：25

共 17 条

[1] BAINES BS, 1982, J PROTEIN CHEM, V1, P120
[2] Bergmann M, 1936, J BIOL CHEM, V115, P593
[3] CHYMOPAPAIN-S IS CHYMOPAPAIN-A
BROCKLEHURST, K
BAINES, BS
SALIH, E
HATZOULIS, C
[J]. BIOCHEMICAL JOURNAL, 1984, 221 (02) : 553 - 554
[4] FRESH NON-FRUIT LATEX OF CARICA-PAPAYA CONTAINS PAPAIN, MULTIPLE FORMS OF CHYMOPAPAIN-A AND PAPAYA PROTEINASE-OMEGA
BROCKLEHURST, K
SALIH, E
MCKEE, R
SMITH, H
[J]. BIOCHEMICAL JOURNAL, 1985, 228 (02) : 525 - 527
[5] BROCKLEHURST K, 1982, METHOD ENZYMOL, V87, P427
[6] A RE-EVALUATION OF THE NOMENCLATURE OF THE CYSTEINE PROTEINASES OF CARICA-PAPAYA AND A RATIONAL BASIS FOR THEIR IDENTIFICATION
BROCKLEHURST, K
SALIH, E
[J]. BIOCHEMICAL JOURNAL, 1983, 213 (02) : 559 - 560
[7] Brocklehurst K., 1981, Topics in Enzyme and Fermentation Biotechnology, V5, P262
[8] EVIDENCE THAT THE ACTIVE-CENTER OF CHYMOPAPAIN-A IS DIFFERENT FROM THE ACTIVE-CENTERS OF SOME OTHER CYSTEINE PROTEINASES AND THAT THE BRONSTED COEFFICIENT (BETA-NUC.) FOR THE REACTIONS OF THIOLATE ANIONS WITH 2,2'-DIPYRIDYL DISULFIDE MAY BE DECREASED BY REAGENT PROTONATION
BROCKLEHURST, K
BAINES, BS
MUSHIRI, MS
[J]. BIOCHEMICAL JOURNAL, 1980, 189 (01) : 189 - 192
[9] 3 STATES CRITERION OF NUCLEOPHILIC REACTIVITY IN CONJUGATE ACID OF AN ANIONIC NUCLEOPHILE AND SOME PROBLEMS OF PROTON PARTITIONING - IMPLICATIONS FOR REACTIONS OF 2,2'-DIPYRIDYL DISULFIDE WITH PAPAIN AND WITH L-ERGOTHIONEINE
BROCKLEHURST, K
[J]. TETRAHEDRON, 1974, 30 (15) : 2397 - 2407
[10] CHYMOPAPAIN - CHROMATOGRAPHIC PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION
BUTTLE, DJ
BARRETT, AJ
[J]. BIOCHEMICAL JOURNAL, 1984, 223 (01) : 81 - 88

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