IDENTIFICATION OF NOVEL MESSENGER-RNAS EXPRESSED IN OLIGODENDROCYTES

被引:18
作者
BABA, H [1 ]
FUSS, B [1 ]
WATSON, JB [1 ]
ZANE, LT [1 ]
MACKLIN, WB [1 ]
机构
[1] UNIV CALIF LOS ANGELES, CTR MED,MENTAL RETARDAT RES CTR,NPI 48241, DEPT PSYCHIAT & BEHAV SCI, LOS ANGELES, CA 90024 USA
关键词
OLIGODENDROCYTES; STEAROYL-COA DESATURASE; SUBTRACTIVE SCREENING; IN SITU HYBRIDIZATION; DYSMYELINATING MUTANTS;
D O I
10.1007/BF00968721
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To identify new proteins, which are expressed in oligodendrocytes and which may have a functional role in myelination, a rat oligodendrocyte cDNA library was screened using differential and subtractive screening techniques. Ten clones that have elevated levels of expression in brain were isolated. Two of these clones were characterized further and one clone, pC26.H2, was found to be closely related to mouse stearoyl-CoA desaturase 2 (SCD2), which catalyzes the synthesis of unsaturated fatly acid. From Northern blot and in situ hybridization studies, SCD2 mRNA was expressed primarily in brain with lower levels found in lung and spleen. In brain sections, SCD2 mRNA was found primarily in oligodendrocytes, although mRNA was detected at a low level in neurons, in particular in Purkinje cells in the cerebellum. Northern blot analysis of the other clone, p973.HB, indicated that it was expressed more selectively in brain. In mixed glial cultures oligodendrocytes were the only cells that expressed this mRNA, whereas in brain, neurons expressed this mRNA at a higher level than in oligodendrocytes. This clone is being actively pursued because of its unique expression exclusively in oligodendrocytes and neurons.
引用
收藏
页码:1091 / 1099
页数:9
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