HOMOGENEOUS AMPEROMETRIC IMMUNOASSAY FOR DIGOXIN

被引：11

作者：

MANNING, P ^{[1
]}

ATHEY, D ^{[1
]}

MCNEIL, CJ ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT CLIN BIOCHEM,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

ANALYTICAL LETTERS | 1994年 / 27卷 / 13期

关键词：

HOMOGENEOUS AMPEROMETRIC IMMUNOASSAY; DIGOXIN; PLATINIZED ACTIVATED CARBON; NADH; EMIT;

D O I：

10.1080/00032719408002653

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A homogeneous spectrophotometric EMIT immunoassay for the measurement of digoxin has been converted to an amperometric immunoassay format capable of analysing digoxin in diluted serum. NADH produced by glucose-6-phosphate dehydrogenase (G6PDH) labelled digoxin was detected amperometrically by its rapid, efficient oxidation at platinized activated carbon electrodes (PACE) poised at +150 mV vs Ag/AgCl. The new amperometric procedure resulted in a simplified assay protocol with a decreased overall assay time, enhanced NADH detection and minimal sample pretreatment.

引用

页码：2443 / 2453

页数：11

共 12 条

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