PHOTOAFFINITY-LABELING OF PROTEIN PHOSPHATASE-2A, THE RECEPTOR FOR A TUMOR PROMOTER OKADAIC ACID, BY [27-H-3]METHYL 7-O-(4-AZIDOBENZOYL)OKADAATE

被引：23

作者：

NISHIWAKI, S

FUJIKI, H

SUGANUMA, M

OJIKA, M

YAMADA, K

SUGIMURA, T

机构：

[1] NATL CANC CTR,RES INST,DIV CANC PREVENT,TSUKIJI 5-1-1,CHUO KU,TOKYO 104,JAPAN

[2] NAGOYA UNIV,FAC SCI,DEPT CHEM,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90544-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of okadaic acid and a protein phosphatase, which is the receptor for a tumor promoter, okadaic acid, was studied. [27-3H]-Methyl 7-O-(4-azidobenzoyl)okadaate was synthesized and protein phosphatase 2A, which consists of two regulatory subunits of 65 kDa and 42 kDa and a catalytic subunit of 37 kDa, was purified from bovine brain. After UV irradiation of the reaction mixture, [3H]methyl 7-O-(4-azidobenzoyl)-okadaate covalently bound to the catalytic subunit, but not to the two regulatory subunits. These results strongly suggest that okadaic acid, a potent inhibitor of protein phosphatases, binds specifically to the catalytic subunit rather than to the other regulatory subunits. © 1990.

引用

页码：1359 / 1364

页数：6

共 21 条

[11]

NISHIWAKI S, 1990, IN PRESS CARCINOGENE

[12] POLYOMA SMALL AND MIDDLE T-ANTIGENS AND SV40 SMALL T-ANTIGEN FORM STABLE COMPLEXES WITH PROTEIN PHOSPHATASE-2A [J].