MOLECULAR-GENETICS OF THE EXTRACELLULAR LIPASE OF PSEUDOMONAS-AERUGINOSA PAO1
被引:75
作者:
WOHLFARTH, S
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机构:Ruhr-Universitat Bochum, Lehrstuhl fur Biologie, der Mikroorganismen
WOHLFARTH, S
HOESCHE, C
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机构:Ruhr-Universitat Bochum, Lehrstuhl fur Biologie, der Mikroorganismen
HOESCHE, C
STRUNK, C
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机构:Ruhr-Universitat Bochum, Lehrstuhl fur Biologie, der Mikroorganismen
STRUNK, C
WINKLER, UK
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机构:Ruhr-Universitat Bochum, Lehrstuhl fur Biologie, der Mikroorganismen
WINKLER, UK
机构:
[1] Ruhr-Universitat Bochum, Lehrstuhl fur Biologie, der Mikroorganismen
来源:
JOURNAL OF GENERAL MICROBIOLOGY
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1992年
/
138卷
关键词:
D O I:
10.1099/00221287-138-7-1325
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
The structural gene (lip A) coding for the extracellular lipase of Pseudomonas aeruginosa PAO1 has been cloned on plasmid pSW118. Nucleotide sequence analysis revealed a gene of 936 bp. lip A codes for a proenzyme of 311 amino acids including a leader sequence of 26 amino acids. The mature protein was predicted to have a M(r) of 30134, an isoelectric point of 5.6, and a consensus sequence (IGHSHGG) typical of lipases. Furthermore it is highly homologous (> 60%) to other lipases from various pseudomonads. The lip A gene failed to hybridize detectably with genomic DNA from other Pseudomonas species except P. alcaligenes, even under relaxed stringency. Located 220 bp downstream of the lip A gene, is an open reading frame (ORF2, lip H) which encodes a hydrophilic protein (283 amino acids; M(r) 33587) that shows some homology to the limA gene product of P. cepacia. In complementation tests of lipase-defective mutants, lip H was shown to be necessary for expression of active extracellular lipase in P. aeruginosa PAO1.