COMPARISON OF REVERSED-PHASE AND ION-PAIR CHROMATOGRAPHY FOR THE DETERMINATION OF STRYCHNINE IN ANIMAL-TISSUES

被引：9

作者：

JOHNSTON, JJ ^{[1
]}

PETTY, EE ^{[1
]}

OKUNO, I ^{[1
]}

NOLTE, DL ^{[1
]}

机构：

[1] USDA,DENVER WILDLIFE RES CTR,OLYMPIA,WA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 670卷 / 01期

关键词：

D O I：

10.1016/0378-4347(95)00156-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion-pair and reversed-phase high-performance liquid chromatography (HPLC) were evaluated for quantification of strychnine in mountain beaver tissues. Retention time shifts hindered strychnine quantification with both HPLC systems. Go-extracted free fatty acids released during storage formed ion-pairs with strychnine, resulting in increased retention by reversed-phase HPLC. Competition with co-extracted basic compounds is likely responsible for the decreased retention of strychnine by ion-pair HPLC. Following an acid-base clean-up, optimal results were obtained with reversed-phase HPLC. Ion-pair chromatography was then used for qualitative confirmation of strychnine residues.

引用

页码：73 / 79

页数：7

共 11 条

[1]

BUSHWAY RJ, 1975, J ASSOC OFF ANA CHEM, V58, P957

[2]

CROUCH MD, 1978, J ASSOC OFF ANA CHEM, V61, P612

[3] RAPID, COMPREHENSIVE SCREENING-PROCEDURE FOR BASIC DRUGS IN BLOOD OR TISSUES BY GAS-CHROMATOGRAPHY [J].