PRODUCTION OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV)-LIKE PARTICLES FROM CELLS INFECTED WITH RECOMBINANT VACCINIA VIRUSES CARRYING THE GAG GENE OF HIV

被引:129
作者
SHIODA, T [1 ]
SHIBUTA, H [1 ]
机构
[1] UNIV TOKYO,INST MED SCI,DEPT VIRAL INFECT,MINATO KU,TOKYO 108,JAPAN
关键词
D O I
10.1016/0042-6822(90)90194-V
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We constructed a recombinant vaccinia virus carrying the entire gag and pol genes of human immunodeficiency virus type t (HIV-1). The main gene product detected in the lysates of infected CV-1 and SW480 cells was the gag precursor protein. However, in the culture fluid of infected SW480 cells, but not of infected CV-1 cells, reverse transcriptase (RT) activity was detected. The highest RT activity was found at a density of 1.15 g/ml and this fraction contained many round particles with diameters of 100-150 nm. In contrast to the infected cell lysates, the particles contained the processed gag and pol proteins, suggesting that particle formation may be a prerequisite for efficient processing of the gag precursor by the HIV protease encoded in the pol gene. Particles were also recovered from the culture fluid of SW480 cells infected with another recombinant vaccinia virus carrying only the gag gene. These particles contained the unprocessed gag precursor, indicating that the gag precursor alone was sufficient for particle production. © 1990.
引用
收藏
页码:139 / 148
页数:10
相关论文
共 47 条
[31]   NUCLEOTIDE-SEQUENCE AND EXPRESSION OF AN AIDS-ASSOCIATED RETROVIRUS (ARV-2) [J].
SANCHEZPESCADOR, R ;
POWER, MD ;
BARR, PJ ;
STEIMER, KS ;
STEMPIEN, MM ;
BROWNSHIMER, SL ;
GEE, WW ;
RENARD, A ;
RANDOLPH, A ;
LEVY, JA ;
DINA, D ;
LUCIW, PA .
SCIENCE, 1985, 227 (4686) :484-492
[32]   ANTIBODIES TO HTLV-III IN SWISS PATIENTS WITH AIDS AND PRE-AIDS AND IN GROUPS AT RISK FOR AIDS [J].
SCHUPBACH, J ;
HALLER, O ;
VOGT, M ;
LUTHY, R ;
JOLLER, H ;
OELZ, O ;
POPOVIC, M ;
SARNGADHARAN, MG ;
GALLO, RC .
NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (05) :265-270
[33]   SEROLOGICAL ANALYSIS OF A SUBGROUP OF HUMAN T-LYMPHOTROPIC RETROVIRUSES (HTLV-III) ASSOCIATED WITH AIDS [J].
SCHUPBACH, J ;
POPOVIC, M ;
GILDEN, RV ;
GONDA, MA ;
SARNGADHARAN, MG ;
GALLO, RC .
SCIENCE, 1984, 224 (4648) :503-505
[34]   HIGH-FREQUENCY OF ABERRANT EXPRESSION OF MOLONEY MURINE LEUKEMIA-VIRUS IN CLONAL INFECTIONS [J].
SHIELDS, A ;
WITTE, ON ;
ROTHENBERG, E ;
BALTIMORE, D .
CELL, 1978, 14 (03) :601-609
[35]   THE MORPHOLOGY OF HUMAN IMMUNODEFICIENCY VIRUS-PARTICLES BY NEGATIVE STAINING ELECTRON-MICROSCOPY [J].
STANNARD, LM ;
VANDERRIET, FDJ ;
MOODIE, JW .
JOURNAL OF GENERAL VIROLOGY, 1987, 68 :919-923
[36]   THE HIV A (SOR) GENE-PRODUCT IS ESSENTIAL FOR VIRUS INFECTIVITY [J].
STREBEL, K ;
DAUGHERTY, D ;
CLOUSE, K ;
COHEN, D ;
FOLKS, T ;
MARTIN, MA .
NATURE, 1987, 328 (6132) :728-730
[37]   HYBRIDIZATION OF DENATURED RNA AND SMALL DNA FRAGMENTS TRANSFERRED TO NITROCELLULOSE [J].
THOMAS, PS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09) :5201-5205
[38]   ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].
TOWBIN, H ;
STAEHELIN, T ;
GORDON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354
[39]   RECOGNITION OF INFLUENZA-A VIRUS NUCLEOPROTEIN BY AN H-2-RESTRICTED CYTO-TOXIC T-CELL CLONE [J].
TOWNSEND, ARM ;
SKEHEL, JJ ;
TAYLOR, PM ;
PALESE, P .
VIROLOGY, 1984, 133 (02) :456-459
[40]   BIOCHEMICAL AND IMMUNOLOGICAL ANALYSIS OF HUMAN IMMUNODEFICIENCY VIRUS GAG GENE PRODUCT-P17 AND PRODUCT-P24 [J].
VERONESE, FD ;
COPELAND, TD ;
OROSZLAN, S ;
GALLO, RC ;
SARNGADHARAN, MG .
JOURNAL OF VIROLOGY, 1988, 62 (03) :795-801