MOLECULAR-DETECTION AND INVIVO ANALYSIS OF THE SPECIFIC T-CELL RESPONSE TO A PROTEIN ANTIGEN

We have analyzed in detail the repertoire of transcripts encoding the V(beta) chains of the T cell receptor and investigated the T cell response of B 10.A mice to pigeon cytochrome c.We were thus able to follow the specific T cell response in vivo after immunization with this protein antigen. The response is first detectable in the draining lymph nodes, then in the spleen and in the blood. It is qualitatively similar in individual animals. It is dominated by a major category of specific T cells harboring a V(beta)3-J(beta)1.2 rearrangement, and a limited and well-defined set of nucleotide sequences, previously found in several specific T cell hybridomas and clones. This predominance is observed from the onset of the immune response strongly suggesting the notion that there is no variation and, therefore, no maturation of the Tcell response in the course of immunization.