DOMAINS OF ESCHERICHIA-COLI GLUTAMINYL-TRANSFER-RNA SYNTHETASE DISORDERED IN THE CRYSTAL-STRUCTURE ARE ESSENTIAL FOR FUNCTION OR STABILITY

被引：2

作者：

CONLEY, J ^{[1
]}

SHERMAN, J ^{[1
]}

THOMANN, HU ^{[1
]}

SOLL, D ^{[1
]}

机构：

[1] YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06520

来源：

NUCLEOSIDES & NUCLEOTIDES | 1994年 / 13卷 / 6-7期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/15257779408012173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deletion analysis of E. coli glutaminyl-tRNA synthetase indicates that the N- and C-termini of the protein, which appear disordered in the crystal structure, are essential for function and stability in vivo. In vitro aminoacylation kinetics of a C-terminal deletion mutant exhibit a sharp reduction in the specificity constant. However, an N-terminal extension is catalytically, if not structurally equivalent to wild-type.

引用

页码：1581 / 1595

页数：15

共 44 条

[11]

HOBEN P, 1985, METHOD ENZYMOL, V113, P55

[12] ANTICODON AND ACCEPTOR STEM NUCLEOTIDES IN TRANSFER RNAGLN ARE MAJOR RECOGNITION ELEMENTS FOR ESCHERICHIA-COLI GLUTAMINYL-TRANSFER RNA-SYNTHETASE [J].

JAHN, M ;

ROGERS, MJ ;

SOLL, D .

NATURE, 1991, 352 (6332) :258-260

[13]

JASIN M, 1985, J BIOL CHEM, V260, P2226

[14] THE GLUTAMINYL-TRANSFER RNA-SYNTHETASE OF ESCHERICHIA-COLI - PURIFICATION, STRUCTURE AND FUNCTION RELATIONSHIP [J].

KERN, D ;

POTIER, S ;

LAPOINTE, J ;

BOULANGER, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 607 (01) :65-80

[15]

KERN D, 1981, EUR J BIOCHEM, V115, P29

[16] SUBUNIT STRUCTURE OF METHIONYL TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI [J].

KOCH, GLE ;

BRUTON, CJ .

FEBS LETTERS, 1974, 40 (01) :180-182

[17] RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION [J].

KUNKEL, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (02) :488-492

[18]

LAPOINTE J, 1992, TRANSLATION EUKARYOT, P35

[19] PROPERTIES OF N-TERMINAL TRUNCATED YEAST ASPARTYL-TRANSFER RNA-SYNTHETASE AND STRUCTURAL CHARACTERISTICS OF THE CLEAVED DOMAIN [J].

LORBER, B ;

MEJDOUB, H ;

REINBOLT, J ;

BOULANGER, Y ;

GIEGE, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 174 (01) :155-161

[20]

LUDMERER SW, 1987, J BIOL CHEM, V262, P10801

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