PREPARATION AND EVALUATION OF AN ONLINE PRECONCENTRATOR FOR CAPILLARY ELECTROPHORESIS

被引：70

作者：

HOYT, AM ^{[1
]}

BEALE, SC ^{[1
]}

LARMANN, JP ^{[1
]}

JORGENSON, JW ^{[1
]}

机构：

[1] UNIV N CAROLINA,DEPT CHEM,CHAPEL HILL,NC 27599

来源：

JOURNAL OF MICROCOLUMN SEPARATIONS | 1993年 / 5卷 / 04期

关键词：

ONLINE PRECONCENTRATION; CAPILLARY ZONE ELECTROPHORESIS; SENSITIVITY ENHANCEMENT; MICROELECTRIC ARC DISCHARGE;

D O I：

10.1002/mcs.1220050405

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The preparation and performance of an on-line preconcentrator for capillary electrophoresis (CE) is described. Borosilicate glass microspheres were dry-packed into the fused silica column terminus and sintered in place with a micro electric arc developed for this application. Reversed-phase LC packing material was slurry-packed onto this frit, in lengths of 1-3 mm. An additional frit of borosilicate beads behind the preconcentrator was used to fix the packed bed in place. The performance of the packed bed preconcentrator for the enhancement of UV and fluorescence detection limits was evaluated. Construction of the micro electric arc device, the packing procedures, and practical applications are described.

引用

页码：325 / 330

页数：6

共 19 条

[11] QUANTITATIVE-ANALYSIS OF LOW-MOLECULAR WEIGHT CARBOXYLIC-ACIDS BY CAPILLARY ZONE ELECTROPHORESIS CONDUCTIVITY DETECTION [J].