FORMYCIN TRIPHOSPHATE AS A PROBE FOR THE ATP BINDING-SITE INVOLVED IN THE ACTIVATION OF GUANYLATE-CYCLASE

被引：3

作者：

CHANG, CH

YU, ZN

SONG, DL

机构：

[1] UNIV TORONTO,DEPT MED GENET,TORONTO M5G 1X5,ONTARIO,CANADA

[2] CASE WESTERN RESERVE UNIV,SCH MED,DEPT PHYSIOL & BIOPHYS,CLEVELAND,OH 44106

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1992年 / 227卷 / 02期

关键词：

FORMYCIN-A TRIPHOSPHATE (FTP); GUANYLATE CYCLASE; ATP; ATRIAL NATRIURETIC FACTOR (ANF); CGMP;

D O I：

10.1016/0922-4106(92)90133-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Formycin A triphosphate (FTP), a fluorescent analog of ATP, slightly increased basal guanylate cyclase activity, but significantly potentiated guanylate cyclase activity stimulated by atrial natriuretic factor (ANF) in rat lung membranes. FTP potentiated ANF-stimulated guanylate cyclase activity with an EC50 at about 90-mu-M and inhibited ATP-stimulated guanylate Cyclase activity with an IC50 at about 100-mu-M. These results indicate that FTP binds more tightly than ATP for the same binding site. Therefore, FTP would be an excellent tool for studying the ATP binding site.

引用

页码：229 / 231

页数：3

共 12 条

[1] EXPLORATION OF THE NUCLEOTIDE BINDING-SITES OF THE ISOLATED ADP/ATP CARRIER PROTEIN FROM BEEF-HEART MITOCHONDRIA .2. PROBING OF THE NUCLEOTIDE SITES BY FORMYCIN TRIPHOSPHATE, A FLUORESCENT TRANSPORTABLE ANALOG OF ATP [J].