CRITHIDIA-FASCICULATA CONTAINS A TRANSCRIBED LEISHMANIAL SURFACE PROTEINASE (GP63) GENE HOMOLOG

被引：47

作者：

INVERSO, JA

MEDINAACOSTA, E

OCONNOR, J

RUSSELL, DG

CROSS, GAM

机构：

[1] ROCKEFELLER UNIV, MOLEC PARASITOL LAB, 1230 YORK AVE, NEW YORK, NY 10021 USA

[2] WASHINGTON UNIV, SCH MED, DEPT MOLEC MICROBIOL, ST LOUIS, MO 63110 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 57卷 / 01期

关键词：

CRITHIDIA-FASCICULATA; GLYCOPROTEIN; LEISHMANIA; METALLOENDOPROTEINASE; PROTEINASE; GP63;

D O I：

10.1016/0166-6851(93)90242-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and analysed a gene from the insect parasite, Crithidia fasciculala, with homology to the gp63 metalloendoproteinase gene of Leishmania. The Crithidia gene homologue is arranged as a multicopy family comprised of approximately 7 genes. The mature transcript is 4.0 kb. The predicted amino acid sequence has significant homology with Leishmania gp63s, contains a zinc-binding motif and a potential site for addition of a glycosylphosphatidylinositol anchor. Demonstration of a gp63 homologue in C. fasciculata, a monogenetic parasite, suggests that the molecule may play a role in parasite survival within the insect gut.

引用

收藏

页码：47 / 54

页数：8

相关论文

共 40 条

[21] ELECTROPHORETIC DETECTION OF TRYPANOSOMA-CRUZI PEPTIDASES [J].

GREIG, S ;

ASHALL, F .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 39 (01) :31-38

[22] MOLECULAR KARYOTYPE AND CHROMOSOMAL LOCALIZATION OF GENES ENCODING 2 MAJOR SURFACE GLYCOPROTEINS, GP63 AND GP46/M2, HSP70, AND BETA-TUBULIN IN CLONED STRAINS OF SEVERAL LEISHMANIA SPECIES [J].

HANEKAMP, T ;

LANGER, PJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 48 (01) :27-38

[23] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[24]

Hoare C. A., 1972, TRYPANOSOMES MAMMALS

[25] A UNIQUE SIGNATURE IDENTIFIES A FAMILY OF ZINC-DEPENDENT METALLOPEPTIDASES [J].

JONGENEEL, CV ;

BOUVIER, J ;

BAIROCH, A .

FEBS LETTERS, 1989, 242 (02) :211-214

[26] EVOLUTION OF PARASITISM - KINETOPLASTID PROTOZOAN HISTORY RECONSTRUCTED FROM MITOCHONDRIAL RIBOSOMAL-RNA GENE-SEQUENCES [J].

LAKE, JA ;

DELACRUZ, VF ;

FERREIRA, PCG ;

MOREL, C ;

SIMPSON, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4779-4783

[27] THE PROMASTIGOTE SURFACE PROTEASE (GP63) OF LEISHMANIA IS EXPRESSED BUT DIFFERENTIALLY PROCESSED AND LOCALIZED IN THE AMASTIGOTE STAGE [J].

MEDINAACOSTA, E ;

KARESS, RE ;

SCHWARTZ, H ;

RUSSELL, DG .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 37 (02) :263-273

[28] STRUCTURALLY DISTINCT GENES FOR THE SURFACE PROTEASE OF LEISHMANIA-MEXICANA ARE DEVELOPMENTALLY REGULATED [J].

MEDINAACOSTA, E ;

KARESS, RE ;

RUSSELL, DG .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 57 (01) :31-46

[29] THE EVOLUTIONARY ORIGIN OF GLYCOSOMES [J].

MICHELS, PAM ;

OPPERDOES, FR .

PARASITOLOGY TODAY, 1991, 7 (05) :105-109

[30] LEISHMANIA GP63 MOLECULE IMPLICATED IN CELLULAR ADHESION LACKS AN ARG-GLY-ASP SEQUENCE [J].

MILLER, RA ;

REED, SG ;

PARSONS, M .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 39 (02) :267-274

← 1 2 3 4 →