LIPASE-CATALYZED INCORPORATION OF N-3 POLYUNSATURATED FATTY-ACIDS INTO VEGETABLE-OILS

The ability of immobilized lipases IM60 from Mucor miehei and SP435 from Candida antarctica to modify the fatty acid composition of selected vegetable oils by incorporation of n-3 polyunsaturated fatty acids into the vegetable oils was studied. The transesterification was carried out in organic solvent with free acid and ethyl esters of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) as acyl donors. With free EPA as acyl donor, IM60 gave higher incorporation of EPA than SP435. However, when ethyl esters of EPA and DHA were the acyl donors, SP435 gave higher incorporation of EPA and DHA than IM60. When IM60 and free acid were used, the addition of 5 mu L water increased EPA incorporation into soybean oil by 4.9%. With ethyl ester of EPA as acyl donor, addition of 2 mu L water increased EPA incorporation by 3.9%. For SP435, addition of water up to 2 mu L resulted in increased EPA incorporation, but the incorporation declined when the added water exceeded this amount. The addition of water increased the EPA incorporation into Trisum 90 after 24 h reaction but not the reaction rate at early stages of the reaction.