CYTOSOLIC CALCIUM OSCILLATIONS INDUCED BY HEPATOCYTE GROWTH-FACTOR (HGF) IN SINGLE FURA-2-LOADED CULTURED-HEPATOCYTES - EFFECTS OF EXTRACELLULAR CALCIUM AND PROTEIN-KINASE-C

Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium. The HGF-induced [Ca2+]i oscillations were suppressed by the pretreatment with phorbol 12-myristate 13-acetate (PMA). Administration of PMA during oscillations also caused their blockade, but the subsequent addition of protein kinase C (PKC) inhibitor H-7 reversed the inhibitory effects of PMA, thereby resulting in the resumption of the oscillatory responses. Moreover, the prior exposure to H-7 caused apparent increases in [Ca2+]i spike peaks elicited by HGF. These results suggest a negative modulation via PKC in HGF-induced repetitive [Ca2+]i transients. The absence of HGF-induced oscillations after the thapsigargin treatment indicates that the agonist-sensitive intracellular Ca2+ pool plays a crucial role in the [Ca2+]oscillations.