AN UNUSUAL GENE CONTAINING A DNAJ N-TERMINAL BOX FLANKS THE PUTATIVE ORIGIN OF REPLICATION OF MYCOPLASMA-GENITALIUM

被引:18
作者
BAILEY, CC
BOTT, KF
机构
[1] UNIV N CAROLINA,DEPT MICROBIOL & IMMUNOL,CHAPEL HILL,NC
[2] UNIV N CAROLINA,DEPT CURRICULUM GENET,CHAPEL HILL,NC
关键词
D O I
10.1128/JB.176.18.5814-5819.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Origins of replication are known to be highly conserved among widely divergent microbial species, with the gene order in those regions being dnaA-dnaN-recF-gyrB. On the basis of sequence identities to entries in GenBank, the gene order of a 6-kb fragment of Mycoplasma genitalium DNA was determined to be dnaN-orf311-gyrB-gyrA-serS, which is structurally similar to the ancestral origin of replication. We have directly linked the dnaN gene to the M. genitalium dnaA gene by PCR amplification. However, we found a novel open reading frame, designated orf311, in place of an expected sequence encoding recF. Orf311 contains a DnaJ box motif at its N terminus, but it has no overall homology to any other protein or sequence in the database. We are unable to detect any recF homolog in M. genitalium by hybridization or during a random sequencing survey of the genome.
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页码:5814 / 5819
页数:6
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