SUPPRESSION OF HEAT-INDUCED HSP70 EXPRESSION BY THE 70-KDA SUBUNIT OF THE HUMAN KU AUTOANTIGEN

被引：54

作者：

LI, GC ^{[1
]}

YANG, SH ^{[1
]}

KIM, D ^{[1
]}

NUSSENZWEIG, A ^{[1
]}

OUYANG, HH ^{[1
]}

WEI, J ^{[1
]}

BURGMAN, P ^{[1
]}

LI, LG ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR,DEPT RADIAT ONCOL,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 10期

关键词：

GENE REGULATION; HEAT SHOCK PROTEIN; TRANSCRIPTION FACTOR; STABLE EXPRESSION;

D O I：

10.1073/pnas.92.10.4512

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Expression of the 70-kDa polypeptide of human Ku autoantigen in rat cells is shown to suppress specifically the induction of hsp70 upon heat shock. Thermal induction of other heat shock proteins is not significantly affected, nor is the state of phosphorylation or the DNA binding ability of the heat shock transcription factor HSF1. These findings support a model in which hsp70 gene expression is controlled by a second regulatory factor in addition to the positive activator HSF1. The Ku autoantigen, or a protein closely related to it, is likely to be involved in the regulation of hsp70 expression.

引用

页码：4512 / 4516

页数：5

共 52 条

[11] DNA-BINDING OF HEAT-SHOCK FACTOR TO THE HEAT-SHOCK ELEMENT IS INSUFFICIENT FOR TRANSCRIPTIONAL ACTIVATION IN MURINE ERYTHROLEUKEMIA-CELLS [J].

HENSOLD, JO ;

HUNT, CR ;

CALDERWOOD, SK ;

HOUSMAN, DE ;

KINGSTON, RE .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (04) :1600-1608

[12] THE REGULATION OF TRANSCRIPTION BY PHOSPHORYLATION [J].

HUNTER, T ;

KARIN, M .

CELL, 1992, 70 (03) :375-387

[13]

Jackson S P, 1992, Trends Cell Biol, V2, P104, DOI 10.1016/0962-8924(92)90014-E

[14] GC BOX BINDING INDUCES PHOSPHORYLATION OF SP1 BY A DNA-DEPENDENT PROTEIN-KINASE [J].

JACKSON, SP ;

MACDONALD, JJ ;

LEESMILLER, S ;

TJIAN, R .

CELL, 1990, 63 (01) :155-165

[15] EFFECT OF SODIUM-SALICYLATE ON THE HUMAN HEAT-SHOCK RESPONSE [J].

JURIVICH, DA ;

SISTONEN, L ;

KROES, RA ;

MORIMOTO, RI .

SCIENCE, 1992, 255 (5049) :1243-1245

[16]

KIM D, 1994, P NATL ACAD SCI USA, V92, P2126

[17] EXPRESSION OF A X-LAEVIS TRANSFER RNA-TYR-GENE IN MAMMALIAN-CELLS [J].

LASKI, FA ;

ALZNERDEWEERD, B ;

RAJBHANDARY, UL ;

SHARP, PA .

NUCLEIC ACIDS RESEARCH, 1982, 10 (15) :4609-4626

[18] HUMAN-CELLS CONTAIN A DNA-ACTIVATED PROTEIN-KINASE THAT PHOSPHORYLATES SIMIAN VIRUS-40 T-ANTIGEN, MOUSE P53, AND THE HUMAN KU-AUTOANTIGEN [J].

LEESMILLER, SP ;

CHEN, YR ;

ANDERSON, CW .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6472-6481

[19] THERMAL RESPONSE OF RAT FIBROBLASTS STABLY TRANSFECTED WITH THE HUMAN 70-KDA HEAT-SHOCK PROTEIN-ENCODING GENE [J].

LI, GC ;

LI, LG ;

LIU, YK ;

MAK, JY ;

CHEN, LL ;

LEE, WMF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (05) :1681-1685

[20] HEAT-SHOCK PROTEIN HSP70 PROTECTS CELLS FROM THERMAL-STRESS EVEN AFTER DELETION OF ITS ATP-BINDING DOMAIN [J].

LI, GC ;

LI, LG ;

LIU, RY ;

REHMAN, M ;

LEE, WMF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2036-2040

← 1 2 3 4 5 6 →