RADIOMETRIC AND FLUORIMETRIC DETERMINATION OF AMINOSILANES AND PROTEIN COVALENTLY BOUND TO THERMALLY PRETREATED GLASS SUBSTRATES

被引：14

作者：

LOCASCIOBROWN, L

PLANT, AL

DURST, RA

BRIZGYS, MV

机构：

[1] NATL INST STANDARDS & TECHNOL, CTR ANALYT CHEM, GAITHERSBURG, MD 20899 USA

[2] CTR ADV RES BIOTECHNOL, ROCKVILLE, MD 20850 USA

来源：

ANALYTICA CHIMICA ACTA | 1990年 / 228卷 / 01期

关键词：

D O I：

10.1016/S0003-2670(00)80485-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Derivatization of solid soda-lime glass spheres with aminosilanes and the stability of these groups near physiological pH in flow streams of aqueous buffered solutions are described. The presence of immobilized and adsorbed amines in the nanomolar range is confirmed by using two independent methods, one based on a radiotracer and the other on the fluorescent marker, fluorescamine. A method for covalently attaching bovine serum albumin to the beads is described. © 1990.

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页码：107 / 116

页数：10

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