ASSIGNMENT OF THE HUMAN PULMONARY SURFACTANT PROTEIN-D GENE (SFTP4) TO 10Q22-Q23 CLOSE TO THE SURFACTANT PROTEIN-A GENE-CLUSTER

被引：53

作者：

KOLBLE, K ^{[1
]}

LU, JH ^{[1
]}

MOLE, SE ^{[1
]}

KALUZ, S ^{[1
]}

REID, KBM ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT PATHOL,CRC,HUMAN CANC GENET RES GRP,CAMBRIDGE CB2 1QP,ENGLAND

来源：

GENOMICS | 1993年 / 17卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1324

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pulmonary surfactant consists of a complex mixture of phospholipids and several proteins essential to normal respiratory function. Two of the surfactant proteins, SP-A and SP-D, appear to have lectin-like activity relevant to the local phagocytic defense. Using polymerase chain reaction (PCR)-based somatic cell hybrid mapping, the human SP-D gene (SFTP4) was assigned to chromosome 10. A regional mapping panel was assembled and characterized using sequence tagged sites for five loci previously mapped to 10q. SFTP4, the SP-A gene (SFTP1), and the microsatellite D10S109 were placed in the interval 10q22-q23. Low-stringency PCR using the SFTP1 primer pair suggested the presence of at least two additional SP-A-related genes in the same region. With the locus for mannose-binding lectin (MBL) at 10q21, this may be indicative of this region's central role in the evolutionary history of carbohydrate-binding proteins containing collagen-like regions. © 1993 Academic Press. All rights reserved.

引用

页码：294 / 298

页数：5