MONOCLONAL-ANTIBODY SANDWICH ENZYME IMMUNOASSAYS FOR DETERMINATION OF GLUTEN IN FOODS

被引：124

作者：

SKERRITT, JH

HILL, AS

机构：

[1] CSIRO Wheat Research Unit, Division of Plant Industry, NSW 2113, P.O. Box 7, North Ryde

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1990年 / 38卷 / 08期

关键词：

D O I：

10.1021/jf00098a029

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The development and properties of two-site (“sandwich”) enzyme immunoassays for gluten in foods, based on monoclonal antibodies to heat-stable ώ-gliadins and related prolamins from wheat, rye, and barley, are described. The complete assay requires under 2 h when precoated microwells are used. The effects of altering component antibodies, gluten extraction conditions, and solvent on assay performance are described. Quantitative results were obtained by using a simple one-step extraction procedure with 40% ethanol over a very wide range of gluten contents (0.015-10%). The method was developed for the quantitative analysis of gluten in virtually all types of foods, whether raw, cooked, or processed. © 1990, American Chemical Society. All rights reserved.

引用

页码：1771 / 1778

页数：8

共 34 条

[1] Development of a rapid enzyme-linked immunosorbent assay (ELISA) for gliadin determination in food [J].

Ayob, M. K. ;

Rittenburg, J. ;

Allen, J. C. ;

Smith, C. J. .

FOOD HYDROCOLLOIDS, 1988, 2 (01) :39-49

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] DETERMINATION OF MAXIMUM AMOUNT OF CEREAL STORAGE PROTEIN IN FOODS - NOTE [J].

CARLSON, TLG ;

ELIASSON, AC ;

NAIR, BM .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1980, 31 (02) :223-224

[4] A RADIOIMMUNOASSAY FOR ALPHA-GLIADIN AND BETA-GLIADIN [J].

CICLITIRA, PJ ;

LENNOX, ES .

CLINICAL SCIENCE, 1983, 64 (06) :655-659

[5]

Cooke WT, 1984, COELIAC DIS

[6]

DONOVAN GR, 1989, J CEREAL SCI, V9, P970

[7] MONOCLONAL-ANTIBODY ELISA TO QUANTITATE WHEAT GLIADIN CONTAMINATION OF GLUTEN-FREE FOODS [J].

FREEDMAN, AR ;

GALFRE, G ;

GAL, E ;

ELLIS, HJ ;

CICLITIRA, PJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 98 (01) :123-127

[8] QUANTITATIVE-DETERMINATION OF WHEAT GLIADINS IN FOODS BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

FRITSCHY, F ;

WINDEMANN, H ;

BAUMGARTNER, E .

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1985, 181 (05) :379-385

[9]

HEUBNER FR, 1970, J AGR FOOD CHEM, V18, P256

[10]

HILL A S, 1989, Food and Agricultural Immunology, V1, P147, DOI 10.1080/09540108909354686

← 1 2 3 4 →