DEVELOPMENT OF AN IMMUNOASSAY USING RECOMBINANT MALTOSE-BINDING PROTEIN STA FUSIONS FOR QUANTITATING ANTIBODY-RESPONSES AGAINST STA, THE HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI

被引：5

作者：

AITKEN, R ^{[1
]}

HIRST, TR ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT GENET,LEICESTER LE1 7RH,ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 03期

关键词：

D O I：

10.1128/JCM.30.3.732-734.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.

引用

页码：732 / 734

页数：3

共 18 条

[1]

DEGUAN C, 1988, GENE, V67, P21

[2]

Dunn, 1986, ELECTROPHORESIS 86, P315

[3] EXPORT AND PROCESSING ANALYSIS OF A FUSION BETWEEN THE EXTRACELLULAR HEAT-STABLE ENTEROTOXIN AND THE PERIPLASMIC B-SUBUNIT OF THE HEAT-LABILE ENTEROTOXIN IN ESCHERICHIA-COLI [J].