DEVELOPMENT OF AN IMMUNOASSAY USING RECOMBINANT MALTOSE-BINDING PROTEIN STA FUSIONS FOR QUANTITATING ANTIBODY-RESPONSES AGAINST STA, THE HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI

被引:5
作者
AITKEN, R [1 ]
HIRST, TR [1 ]
机构
[1] UNIV LEICESTER,DEPT GENET,LEICESTER LE1 7RH,ENGLAND
关键词
D O I
10.1128/JCM.30.3.732-734.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.
引用
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页码:732 / 734
页数:3
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