DEVELOPMENT OF AN IMMUNOASSAY USING RECOMBINANT MALTOSE-BINDING PROTEIN STA FUSIONS FOR QUANTITATING ANTIBODY-RESPONSES AGAINST STA, THE HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI
A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.