AMPLIFIED VITAMIN-C SENSOR-BASED ON THE CYCLIC REACTION BETWEEN L-ASCORBIC-ACID AND DEHYDROASCORBIC ACID

被引：20

作者：

UCHIYAMA, S

HASEBE, Y

SUZUKI, S

机构：

[1] Department of Environmental Engineering, Saitama Institute of Technology, Saitama, 369-02, 1690 Fusaiji, Okabe

来源：

ELECTROANALYSIS | 1993年 / 5卷 / 08期

关键词：

ASCORBIC ACID; AMPLIFICATION; BIOSENSOR;

D O I：

10.1002/elan.1140050805

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The current response of L-ascorbic acid at an ascorbate-oxidase membrane electrode (detecting the decrease of oxygen) has been amplified by adding dithiothreithol into the sample solution. The signal amplification is based on the cyclic reaction between L-ascorbic acid and dehydroascorbic acid, which leads to the regeneration of L-ascorbic acid. The amplification factor was increased up to 22.5 (at pH -7.5) when 5 x 10(-3) M dithiothreithol solution was used. This amplification system enables the determination of not only L-ascorbic acid but dehydroascorbic acid, and the detection limit for vitamin C was found to be 2 X 10(-7) M.

引用

页码：653 / 655

页数：3

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