BIOSYNTHESIS OF THYROTROPIN RELEASING FACTOR - EFFECT OF RNASE TREATMENT

被引：3

作者：

GRIMMJORGENSEN, Y ^{[1
]}

MCKELVY, JF ^{[1
]}

机构：

[1] UNIV CONNECTICUT, CTR HLTH, DEPT ANAT, FARMINGTON, CT 06032 USA

来源：

BRAIN RESEARCH BULLETIN | 1976年 / 1卷 / 02期

关键词：

D O I：

10.1016/0361-9230(76)90066-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The role of RNA in the in vitro biosynthesis of thyrotropin releasing factor (TRF) was investigated. The biosynthesis of TRF was assessed by measuring the ability of a 1000 g supernatant fraction of newt brain origin to incorporate (3H)proline into (3H)TRF. Measurable amounts of (3H)TRF are synthesized by newt brain homogenates, preincubation of these homogenates with protease-free RNase completely abolishes (3H)TRF synthesis, while preincubation of homogenates with inactivated RNase allows for (3H)TRF synthesis to proceed. The presence of intact RNA is mandatory for TRF biosynthesis by newt brain homogenates.

引用

页码：171 / 175

页数：5

共 26 条

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