MONOCLONAL-ANTIBODIES TO GLUTATHIONE-S-TRANSFERASE PI-IMMUNOHISTOCHEMICAL ANALYSIS OF HUMAN TISSUES AND CANCERS

被引：59

作者：

KANTOR, RRS

GIARDINA, SL

BARTOLAZZI, A

TOWNSEND, AJ

MYERS, CE

COWAN, KH

LONGO, DL

NATALI, PG

机构：

[1] REGINA ELENA INST CANC RES,DEPT IMMUNOL,ROME,ITALY

[2] NCI,DIV CANC TREATMENT,MED BRANCH,BETHESDA,MD 20892

[3] NCI,DIV CANC TREATMENT,BIOL RESPONSE MODIFIERS PROGRAM,FREDERICK,MD 21701

来源：

INTERNATIONAL JOURNAL OF CANCER | 1991年 / 47卷 / 02期

关键词：

D O I：

10.1002/ijc.2910470206

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S-transferase (GST-pi). MAb AGST 1 can inhibit 50-70% of GST-pi enzymatic activity and reacts with a 3-dimensional epitope which includes a putative glutathione binding site on GST-pi. A sandwich enzyme-immunoassay established using MAb AGST 1 and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST-pi levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro- and chondrosarcomas.

引用

页码：193 / 201

页数：9

共 52 条

[41]

SHERMAN M, 1983, HEPATOLOGY, V3, P170

[42]

SHIRATORI Y, 1987, CANCER RES, V47, P6806

[43] PURIFICATION AND SUBUNIT-STRUCTURAL AND IMMUNOLOGICAL CHARACTERIZATION OF 5 GLUTATHIONE S-TRANSFERASES IN HUMAN-LIVER, AND THE ACIDIC FORM AS A HEPATIC TUMOR-MARKER [J].