HYPERVARIABLE EPITOPE CONSTRUCTS AS A MEANS OF ACCOUNTING FOR EPITOPE VARIABILITY

被引：20

作者：

ANDERSON, DE

MALLEY, A

BENJAMINI, E

GARDNER, MB

TORRES, JV

机构：

[1] UNIV CALIF DAVIS,SCH MED,DEPT MICROBIOL & IMMUNOL,DAVIS,CA 95616

[2] UNIV CALIF DAVIS,SCH MED,DEPT PATHOL,DAVIS,CA 95616

[3] OREGON REG PRIMATE RES CTR,BEAVERTON,OR 97006

来源：

VACCINE | 1994年 / 12卷 / 08期

关键词：

HYPERVARIABLE EPITOPE CONSTRUCTS; EPITOPE VARIABILITY; SIMIAN IMMUNODEFICIENCY VIRUS;

D O I：

10.1016/0264-410X(94)90225-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Epitope variability is one of the greatest obstacles to development of synthetic peptide vaccines. Based on a recently described hypervariable epitope (aa 414-434) on the envelope glycoprotein (gp130) to simian immunodeficiency virus (SIVmac142), we have developed a novel approach to account for epitope variability. We have prepared, in a single synthesis, a cocktail of peptides, designated a hypervariable epitope construct (HEC), which collectively represent all the in vivo variability seen in an epitope. The HEC represents permutations of amino acid substitutions found in the epitope and has been able to induce antibodies with enhanced binding to native SIV and broad immunoreactivity to related epitope analogues.

引用

页码：736 / 740

页数：5

共 22 条

[11] IDENTIFICATION OF OVERLAPPING HLA CLASS I-RESTRICTED CYTOTOXIC T-CELL EPITOPES IN A CONSERVED REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN - DEFINITION OF MINIMUM EPITOPES AND ANALYSIS OF THE EFFECTS OF SEQUENCE VARIATION [J].