A NEW MYXOCOCCUS-XANTHUS GENE-CLUSTER FOR THE BIOSYNTHESIS OF THE ANTIBIOTIC SAFRAMYCIN MX1 ENCODING A PEPTIDE SYNTHETASE

被引：55

作者：

POSPIECH, A ^{[1
]}

CLUZEL, B ^{[1
]}

BIETENHADER, J ^{[1
]}

SCHUPP, T ^{[1
]}

机构：

[1] CIBA GEIGY AG, BIOTECHNOL RES, CH-4002 BASEL, SWITZERLAND

来源：

MICROBIOLOGY-SGM | 1995年 / 141卷

关键词：

MYXOCOCCUS XANTHUS; SAFRAMYCIN MX1; ANTIBIOTIC; PEPTIDE SYNTHETASE;

D O I：

10.1099/13500872-141-8-1793

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene cluster for the biosynthesis of the heterocyclic quinone antibiotic saframycin Mx1 of Myxococcus xanthus DM504/15 was inactivated and tagged by Tn5 insertions. The tagged genes were cloned in Escherichia coli and used to select overlapping cosmid clones spanning 58 kb of the M. xanthus genome. Gene disruption experiments defined a greater than or equal to 18 kb contiguous DNA region involved in saframycin biosynthesis. Sequencing of part of this region revealed a large ORF containing two 600-amino-acid domains with similarity to peptide synthetase amino-acid-activating sequences, suggesting that saframycin Mx1 is synthesized by a nonribosomal multienzyme complex, similar to other bioactive peptides.

引用

页码：1793 / 1803

页数：11

共 49 条

[1] GENETIC-ANALYSIS OF ABSB, A STREPTOMYCES-COELICOLOR LOCUS INVOLVED IN GLOBAL ANTIBIOTIC REGULATION [J].

ADAMIDIS, T ;

CHAMPNESS, W .

JOURNAL OF BACTERIOLOGY, 1992, 174 (14) :4622-4628

[2] MUTATIONS IN A NEW STREPTOMYCES-COELICOLOR LOCUS WHICH GLOBALLY BLOCK ANTIBIOTIC BIOSYNTHESIS BUT NOT SPORULATION [J].

ADAMIDIS, T ;

RIGGLE, P ;

CHAMPNESS, W .

JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :2962-2969

[3]

ARAI T, 1977, J ANTIBIOT, V30, P1015

[4]

ARAI T, 1980, GANN, V71, P790

[5] DIRECTED BIOSYNTHESIS OF NEW SAFRAMYCIN DERIVATIVES WITH RESTING CELLS OF STREPTOMYCES-LAVENDULAE [J].

ARAI, T ;

YAZAWA, K ;

TAKAHASHI, K ;

MAEDA, A ;

MIKAMI, Y .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 28 (01) :5-11

[6] SEQUENCE AND ANALYSIS OF THE GENETIC-LOCUS RESPONSIBLE FOR SURFACTIN SYNTHESIS IN BACILLUS-SUBTILIS [J].

COSMINA, P ;

RODRIGUEZ, F ;

DEFERRA, F ;

GRANDI, G ;

PEREGO, M ;

VENEMA, G ;

VANSINDEREN, D .

MOLECULAR MICROBIOLOGY, 1993, 8 (05) :821-831

[7] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[8] AMINO-ACYLATION SITE MUTATIONS IN AMINO ACID-ACTIVATING DOMAINS OF SURFACTIN SYNTHETASE - EFFECTS ON SURFACTIN PRODUCTION AND COMPETENCE DEVELOPMENT IN BACILLUS-SUBTILIS [J].

DSOUZA, C ;

NAKANO, MM ;

CORBELL, N ;

ZUBER, P .

JOURNAL OF BACTERIOLOGY, 1993, 175 (11) :3502-3510

[9]

GERTH K, 1982, J ANTIBIOT, V35, P1454, DOI 10.7164/antibiotics.35.1454

[10] ANALYSIS OF CORE SEQUENCES IN THE D-PHE ACTIVATING DOMAIN OF THE MULTIFUNCTIONAL PEPTIDE SYNTHETASE TYCA BY SITE-DIRECTED MUTAGENESIS [J].

GOCHT, M ;

MARAHIEL, MA .

JOURNAL OF BACTERIOLOGY, 1994, 176 (09) :2654-2662

← 1 2 3 4 5 →