HUMAN CHROMOSOME-SPECIFIC CDNA LIBRARIES - NEW TOOLS FOR GENE IDENTIFICATION AND GENOME ANNOTATION

被引：16

作者：

DELMASTRO, RG

WANG, LP

SIMMONS, AD

GALLARDO, TD

CLINES, GA

ASHLEY, JA

HILLIARD, CJ

WASMUTH, JJ

MCPHERSON, JD

LOVETT, M

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235

[2] UNIV TEXAS,SW MED CTR,MCDERMOTT CTR HUMAN GROWTH & DEV,DALLAS,TX 75235

[3] UNIV CALIF IRVINE,DEPT BIOL CHEM,IRVINE,CA 92717

[4] UNIV CALIF IRVINE,CTR HUMAN GENOME,IRVINE,CA 92717

来源：

GENOME RESEARCH | 1995年 / 5卷 / 02期

关键词：

D O I：

10.1101/gr.5.2.185

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from Five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains similar to 20% of the similar to 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.

引用

页码：185 / 194

页数：10

共 43 条

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