ANALYSIS OF 6 VNTR LOCI BY MULTIPLEX PCR AND AUTOMATED FLUORESCENT DETECTION

被引：25

作者：

TULLY, G

SULLIVAN, KM

GILL, P

机构：

[1] Central Research and Support Establishment, Home Office Forensic Science Service, Aldermaston, Reading, RG7 4PN, Berkshire

来源：

HUMAN GENETICS | 1993年 / 92卷 / 06期

关键词：

D O I：

10.1007/BF00420938

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

引用

页码：554 / 562

页数：9

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