APPLICATION OF PCR FOR DETECTION OF TOXIGENIC CORYNEBACTERIUM-DIPHTHERIAE STRAINS ISOLATED DURING THE RUSSIAN DIPHTHERIA EPIDEMIC, 1990 THROUGH 1994

被引：33

作者：

MIKHAILOVICH, VM

MELNIKOV, VG

MAZUROVA, IK

WACHSMUTH, IK

WENGER, JD

WHARTON, M

NAKAO, H

POPOVIC, T

机构：

[1] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV BACTERIAL & MYCOT DIS,ATLANTA,GA 30333

[2] GN GABRICHEVSKII EPIDEMIOL & MICROBIOL RES INST,RUSSIAN FED REFERENCE LAB DIPHTHERIA,MOSCOW,RUSSIA

[3] CTR DIS CONTROL & PREVENT,NATL IMMUNIZATION PROGRAM,DIV EPIDEMIOL & SURVEILLANCE,ATLANTA,GA 30333

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 11期

关键词：

D O I：

10.1128/JCM.33.11.3061-3063.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A total of 250 Corynebacterium diphtheriae isolates from clinical cases and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigenic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete concordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinical laboratories.

引用

页码：3061 / 3063

页数：3

共 11 条

[1]

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[2]

EFSTRATIOU A, COMMUNICATION

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