USE OF IN-VIVO COMPLEMENTATION IN MYCOBACTERIUM-TUBERCULOSIS TO IDENTIFY A GENOMIC FRAGMENT ASSOCIATED WITH VIRULENCE

被引:82
作者
PASCOPELLA, L
COLLINS, FM
MARTIN, JM
LEE, MH
HATFULL, GF
STOVER, CK
BLOOM, BR
JACOBS, WR
机构
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED, HOWARD HUGHES MED INST, DEPT MICROBIOL & IMMUNOL, BRONX, NY 10461 USA
[2] TRUDEAU INST INC, SARANAC LAKE, NY 12983 USA
[3] UNIV PITTSBURGH, DEPT BIOL SCI, PITTSBURGH, PA 15260 USA
[4] MEDIMMUNE INC, GAITHERSBURG, MD 20878 USA
关键词
D O I
10.1128/IAI.62.4.1313-1319.1994
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.
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页码:1313 / 1319
页数:7
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