PERSISTING IN-VITRO MOTILITY OF ACTIN-FILAMENTS AT NANOMOLAR ATP CONCENTRATIONS AFTER ATP PRETREATMENT

被引：7

作者：

KELLERMAYER, MSZ ^{[1
]}

HINDS, TR ^{[1
]}

POLLACK, GH ^{[1
]}

机构：

[1] UNIV WASHINGTON, CTR BIOENGN, WD-12, SEATTLE, WA 98195 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1995年 / 1229卷 / 01期

关键词：

ACTIN FILAMENT; HEAVY MEROMYOSIN; MOTILITY ASSAY; IN VITRO; ATP; ENERGY PRODUCTION; UNEXPLAINED;

D O I：

10.1016/0005-2728(94)00191-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Muscle contraction is driven by the relative sliding of actin- and myosin-containing filaments. This process can be reconstituted in in vitro motility assays, where fluorescent actin filaments travel over a lawn of myosin heads. The velocity of the traveling actin filaments has been shown to depend strongly on the free adenosine triphosphate (ATP) concentration, but below 2-4 mu M ATP, filament movement is consistently abolished. Here we report that after a brief exposure of actomyosin to 1 mM ATP, actin filament motility persists down to nanomolar ATP concentrations.

引用

页码：89 / 95

页数：7

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