PERSISTING IN-VITRO MOTILITY OF ACTIN-FILAMENTS AT NANOMOLAR ATP CONCENTRATIONS AFTER ATP PRETREATMENT

被引：7

作者：

KELLERMAYER, MSZ ^{[1
]}

HINDS, TR ^{[1
]}

POLLACK, GH ^{[1
]}

机构：

[1] UNIV WASHINGTON, CTR BIOENGN, WD-12, SEATTLE, WA 98195 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1995年 / 1229卷 / 01期

关键词：

ACTIN FILAMENT; HEAVY MEROMYOSIN; MOTILITY ASSAY; IN VITRO; ATP; ENERGY PRODUCTION; UNEXPLAINED;

D O I：

10.1016/0005-2728(94)00191-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Muscle contraction is driven by the relative sliding of actin- and myosin-containing filaments. This process can be reconstituted in in vitro motility assays, where fluorescent actin filaments travel over a lawn of myosin heads. The velocity of the traveling actin filaments has been shown to depend strongly on the free adenosine triphosphate (ATP) concentration, but below 2-4 mu M ATP, filament movement is consistently abolished. Here we report that after a brief exposure of actomyosin to 1 mM ATP, actin filament motility persists down to nanomolar ATP concentrations.

引用

页码：89 / 95

页数：7

共 37 条

[21]

Yonkoski, Soane, Materials Science of High Temperature Polymers for Microelectronics Symposium, pp. 259-264, (1991)

[22]

Kishino, Yanagida, Nature, 334, pp. 74-76, (1998)

[23]

Uyeda, Kron, Spudich, J. Mol. Biol., 214, pp. 699-710, (1990)

[24]

Wu, Hinds, Vincenzi, Biochim. Biophys. Acta, 1106, pp. 56-62, (1992)

[25]

Lymn, Taylor, Biochemistry, 10, pp. 4617-4624, (1971)

[26]

Sowerby, Seehra, Lee, Bagshaw, J. Mol. Biol., 234, pp. 114-123, (1993)

[27]

bauer, Baumann, Trommer, FEBS Lett., 313, pp. 288-290, (1992)

[28]

Reuben, Brandt, Berman, Grundfest, J. Gen. Physiol., 57, pp. 385-407, (1971)

[29]

Goldman, Hibberd, Trentham, J. Physiol. (London), 354, pp. 577-604, (1984)

[30]

Ushitani, Iwamoto, Sugi, J. Muscle Res. Cell Motil., 15, pp. 354-355, (1994)

← 1 2 3 4 →