INFLUENCE OF HORMONES ON THE INHIBITORY ACTIVITY OF OOCYTE MATURATION PRESENT IN CONDITIONED MEDIA OF PORCINE GRANULOSA-CELLS

This study examines the influence of follicular maturation as well as the role of various hormones upon the secretion of an oocyte maturation inhibitor (OMI) from porcine granulosa cells incubated in vitro. The OMI substance, secreted into the media by granulosa cells, is present in a low MW fraction (< 10,000 daltons) similar to that found in follicular fluid of porcine antral follicles. Also, as follicular development progresses, the granulosa cells lose their ability to secrete OMI. More importantly, hormones appear to regulate OMI secretion: FSH stimulates OMI secretion and androgens inhibit OMI secretion. These data provide evidence for the proposal of the following hypothesis concerning hormones regulation of oocyte meiosis by OMI in the porcine follicle: Whether the oocyte resumes meiosis, either during atresia or ovulation, is dependent upon the proper milieu of gonadotropins, cAMP, and steroids within the microenvironment of the follicular compartment. The cellular interactions of these hormones, particularly FSH and androgens, control the amount of OMI (and possibly other intrafollicular factors) secreted in the follicle, which may be involved in either maintaining the immature state or permitting meiotic maturation.