FLAVOBACTERIUM-MENINGOSEPTICUM PEPTIDE - N-GLYCOSIDASE - INFLUENCE OF IONIC-STRENGTH ON ENZYMATIC-ACTIVITY

被引：2

作者：

GOSSELIN, S

MARTIN, BM

MURRAY, GJ

VISWANATHA, T

机构：

[1] UNIV WATERLOO, GUELPH WATERLOO CTR GRAD WORK CHEM, WATERLOO N2L 3G1, ONTARIO, CANADA

[2] NIMH, CLIN NEUROSCI BRANCH, BETHESDA, MD 20892 USA

[3] NINCDS, MOLEC GENET LAB, BETHESDA, MD 20892 USA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 24卷 / 1-2期

基金：

加拿大自然科学与工程研究理事会;

关键词：

PEPTIDE; N-GLYCOSIDASE; GLYCOPROTEIN; DEGLYCOSYLATION; IONIC STRENGTH;

D O I：

10.1016/0165-022X(92)90048-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flavobacterium meningosepticum peptide: N-glycosidase-mediated deglycosylation of N-linked glycan strands of glycoproteins has been found to be strongly influenced by the ionic strength of the assay medium. By use of a modification of a previously published assay procedure for quantitative analysis of glycan release we have been able to improve reproducibility and thus to compare the extent of deglycosylation achieved under a variety of conditions of ionic strength. We have observed that enzyme activity is adversely affected by high ionic strength buffers such as those recommended for deglycosylation of various glycoproteins and recommend the use of low ionic strength buffers for routine use.

引用

页码：71 / 79

页数：9

共 26 条

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