ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .6. MICROELECTRODES FOR THE DETECTION OF L-LACTATE BASED ON FLAVOCYTOCHROME B(2) IMMOBILIZED IN A POLY(PHENOL) FILM

被引：32

作者：

BARTLETT, PN

CARUANA, DJ

机构：

来源：

ANALYST | 1994年 / 119卷 / 02期

关键词：

FLAVOCYTOCHROME B(2); MICROELECTRODE; POLY(PHENOL); MEDIATION; L-LACTATE DETERMINATION;

D O I：

10.1039/an9941900175

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The adsorption of flavocytochrome b(2) at a 25 mu m diameter platinum microelectrode, followed by immobilization in an electrochemically polymerized phenol film, was found to be a reproducible method for the fabrication of microelectrodes responsive to L-lactate. Fabrication of enzyme microelectrodes in this manner is simple and produces electrodes that respond rapidly to L-lactate. The immobilization in the poly(phenol) film has no effect on the specificity of the enzyme for L-lactate over beta-chlorolactate and reduces the direct electrochemical interference from uric acid, acetoamidophenol and ascorbate. The stability of the resulting enzyme electrode is discussed.

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页码：175 / 180

页数：6

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