MAPPING OF A HUMAN BRAIN VOLTAGE-GATED CALCIUM-CHANNEL TO HUMAN CHROMOSOME-12P13-PTER

被引：14

作者：

SUN, W

MCPHERSON, JD

HOANG, DQ

WASMUTH, JJ

EVANS, GA

MONTAL, M

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

[2] UNIV CALIF IRVINE,DEPT BIOL CHEM,IRVINE,CA 92717

[3] SALK INST BIOL STUDIES,MOLEC GENET LAB,SAN DIEGO,CA 92138

来源：

GENOMICS | 1992年 / 14卷 / 04期

关键词：

D O I：

10.1016/S0888-7543(05)80135-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter. © 1992 Academic Press, Inc. All rights reserved.

引用

页码：1092 / 1094

页数：3

共 17 条

[1] ISOLATION OF A NOVEL MILDLY REPETITIVE DNA-SEQUENCE THAT IS PREDOMINANTLY LOCATED AT THE TERMINUS OF THE SHORT ARM OF CHROMOSOME-4 NEAR THE HUNTINGTON DISEASE GENE [J].