THE CELL-ENVELOPE STRUCTURE OF THE LIPOPOLYSACCHARIDE-LACKING GRAM-NEGATIVE BACTERIUM SPHINGOMONAS-PAUCIMOBILIS

被引:136
作者
KAWASAKI, S
MORIGUCHI, R
SEKIYA, K
NAKAI, T
ONO, E
KUME, K
KAWAHARA, K
机构
[1] KITASATO INST,DEPT BACTERIOL,5-9-1 SHIROKANE,MINATO KU,TOKYO 108,JAPAN
[2] KITASATO INST,DEPT PATHOL,TOKYO 108,JAPAN
[3] KITASATO UNIV,SCH PHARMACEUT SCI,DEPT MICROBIOL,MINATO KU,TOKYO 108,JAPAN
[4] KITASATO INST,VET SCI RES CTR,TOKYO 108,JAPAN
关键词
D O I
10.1128/JB.176.2.284-290.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipopolysaccharide-lacking gram-negative bacterium. The low-density fraction showed many minor bands of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and NADH oxidase activity was localized in this fraction. Combined with morphological data of vesicles formed by these membrane fractions, the high-density and low-density fractions were proposed to be an outer membrane and a cytoplasmic membrane, respectively. The localization of the glycosphingolipid was investigated also by means of immunoelectron microscopic analysis using a glycosphingolipid-specific antibody. The glycosphingolipid was shown to localize at the cell envelope, and the antigenic sugar portion was exposed to the bacterial cell surface. From these results the glycosphingolipid was assumed to have a function similar to that of the lipopolysaccharide of other gram-negative bacteria.
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页码:284 / 290
页数:7
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