HYPERSENSITIVE SITE-4 OF THE HUMAN BETA GLOBIN LOCUS-CONTROL REGION

被引：127

作者：

PRUZINA, S ^{[1
]}

HANSCOMBE, O ^{[1
]}

WHYATT, D ^{[1
]}

GROSVELD, F ^{[1
]}

PHILIPSEN, S ^{[1
]}

机构：

[1] NATL INST MED RES,RIDGEWAY,MILL HILL,LONDON NW7 1AA,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 07期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/19.7.1413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Locus Control Region (LCR) of the human beta-globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

引用

页码：1413 / 1419

页数：7

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