Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

被引：449

作者：

Gardiner, Chris ^{[1
]}

Ferreira, Yannick J. ^{[1
]}

Dragovic, Rebecca A. ^{[1
]}

Redman, Christopher W. G. ^{[1
]}

Sargent, Ian L. ^{[1
]}

机构：

[1] Univ Oxford, Nuffield Dept Obstet & Gynaecol, John Radcliffe Hosp, Womens Ctr, Level 3, Oxford OX3 9DU, England

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2013年 / 2卷 / 01期

基金：

英国惠康基金;

关键词：

extracellular vesicles; nanoparticle tracking analysis; light scattering; standardisation;

D O I：

10.3402/jev.v2i0.19671

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

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页数：11

相关论文

共 18 条

[1]

Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability [J].

Aleman, M. M. ;

Gardiner, C. ;

Harrison, P. ;

Wolberg, A. S. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2011, 9 (11) :2251-2261

[2]

Measurement of circulating cell-derived microparticles by flow cytometry: Sources of variability within the assay [J].

Ayers, Lisa ;

Kohler, Malcolm ;

Harrison, Paul ;

Sargent, Ian ;

Dragovic, Rebecca ;

Schaap, Marianne ;

Nieuwland, Rienk ;

Brooks, Susan A. ;

Ferry, Berne .

THROMBOSIS RESEARCH, 2011, 127 (04) :370-377

[3]

Carr R, 2009, EUROPEAN J PARENTERA, V14, P45

[4]

A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer [J].

Chandler, W. L. ;

Yeung, W. ;

Tait, J. F. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2011, 9 (06) :1216-1224

[5]

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis [J].

Dragovic, Rebecca A. ;

Gardiner, Christopher ;

Brooks, Alexandra S. ;

Tannetta, Dionne S. ;

Ferguson, David J. P. ;

Hole, Patrick ;

Carr, Bob ;

Redman, Christopher W. G. ;

Harris, Adrian L. ;

Dobson, Peter J. ;

Harrison, Paul ;

Sargent, Ian L. .

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2011, 7 (06) :780-788

[6]

Exosome-mediated delivery of siRNA in vitro and in vivo [J].

El-Andaloussi, Samir ;

Lee, Yi ;

Lakhal-Littleton, Samira ;

Li, Jinghuan ;

Seow, Yiqi ;

Gardiner, Chris ;

Alvarez-Erviti, Lydia ;

Sargent, Ian L. ;

Wood, Matthew J. A. .

NATURE PROTOCOLS, 2012, 7 (12) :2112-2126

[7]

Critical Evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the Measurement of Nanoparticles and Protein Aggregates [J].

Filipe, Vasco ;

Hawe, Andrea ;

Jiskoot, Wim .

PHARMACEUTICAL RESEARCH, 2010, 27 (05) :796-810

[8]

Nanoparticle analysis of circulating cell-derived vesicles in ovarian cancer patients [J].

Gercel-Taylor, Cicek ;

Atay, Safinur ;

Tullis, Richard H. ;

Kesimer, Mehmet ;

Taylor, Douglas D. .

ANALYTICAL BIOCHEMISTRY, 2012, 428 (01) :44-53

[9]

Improved Flow Cytometric Assessment Reveals Distinct Microvesicle (Cell-Derived Microparticle) Signatures in Joint Diseases [J].

Gyoergy, Bence ;

Szabo, Tamas G. ;

Turiak, Lilla ;

Wright, Matthew ;

Herczeg, Petra ;

Ledeczi, Zsigmond ;

Kittel, Agnes ;

Polgar, Anna ;

Toth, Kalman ;

Derfalvi, Beata ;

Zelenak, Gergo ;

Boeroecz, Istvan ;

Carr, Bob ;

Nagy, Gyoergy ;

Vekey, Karoly ;

Gay, Steffen ;

Falus, Andras ;

Buzas, Edit I. .

PLOS ONE, 2012, 7 (11)

[10]

Impact of pre-analytical parameters on the measurement of circulating microparticles: towards standardization of protocol [J].

Lacroix, R. ;

Judicone, C. ;

Poncelet, P. ;

Robert, S. ;

Arnaud, L. ;

Sampol, J. ;

Dignat-George, F. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2012, 10 (03) :437-446