DIRECT ANALYSIS OF BACTERIAL FATTY-ACIDS BY CURIE-POINT PYROLYSIS TANDEM MASS-SPECTROMETRY

被引：69

作者：

DELUCA, S ^{[1
]}

SARVER, EW ^{[1
]}

HARRINGTON, PD ^{[1
]}

VOORHEES, KJ ^{[1
]}

机构：

[1] COLORADO SCH MINES,GOLDEN,CO 80401

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 14期

关键词：

D O I：

10.1021/ac00213a022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Although pyrolysis-mass spectrometry (Py-MS) has been used for bacterial taxonomy, many of the mass spectral peaks used for discrimination of organisms have not been correlated to known biomolecules. This work presents the discrimination of five bacterial species based on Py-MS patterns containing only peaks that can be correlated to fatty acids and fatty acid derivatives. These correlations were confirmed by pyrolysis-tandem mass spectrometry of authentic standards and the organisms. The pattern recognition procedures used gave better results when only the fatty acid peaks were used in the analysis than when full spectra were used. © 1990, American Chemical Society. All rights reserved.

引用

页码：1465 / 1472

页数：8

共 17 条

[1] IDENTIFICATION OF BACTERIA USING MASS-SPECTROMETRY [J].