THE 1ST GENE IN THE ESCHERICHIA-COLI SECA OPERON, GENE-X, ENCODES A NONESSENTIAL SECRETORY PROTEIN

被引：37

作者：

RAJAPANDI, T ^{[1
]}

DOLAN, KM ^{[1
]}

OLIVER, DB ^{[1
]}

机构：

[1] SUNY STONY BROOK, DEPT MICROBIOL, STONY BROOK, NY 11794 USA

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 22期

关键词：

D O I：

10.1128/jb.173.22.7092-7097.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

TnphoA insertions in the first gene of the Escherichia coli secA operon, gene X, were isolated and analyzed. Studies of the Gene X-PhoA fusion proteins showed that gene X encodes a secretory protein, since the fusion proteins possessed normal alkaline phosphatase activity and a substantial portion of this activity was found in the periplasm. In addition, the Gene X-PhoA fusion proteins were initially synthesized with a cleavable signal peptide. A gene X::TnphoA insertion was used to construct a strain containing a disrupted chromosomal copy of gene X. Analysis of this strain indicated that gene X is nonessential for cell growth and viability and does not appear to play an essential role in the process of protein export.

引用

页码：7092 / 7097

页数：6

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