REVERSIBLE EXPRESSION OF SM ALPHA-ACTIN PROTEIN AND SM ALPHA-ACTIN MESSENGER-RNA IN CLONED CEREBRAL ENDOTHELIAL-CELLS

被引：22

作者：

AMBERGER, A

BAUER, H

TONTSCH, U

GABBIANI, G

KOCHER, O

BAUER, HC

机构：

[1] AUSTRIAN ACAD SCI,INST MOLEK BIOL,BILLROTHSTR 11,A-5020 SALZBURG,AUSTRIA

[2] SALZBURG UNIV,INST ZOOL,A-5020 SALZBURG,AUSTRIA

[3] UNIV GENEVA,DEPT PATHOL,CH-1211 GENEVA 4,SWITZERLAND

[4] YALE UNIV,SCH MED,DEPT PATHOL,NEW HAVEN,CT 06510

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

CEREBRAL ENDOTHELIAL CELL; PHENOTYPIC SWITCH; SMOOTH MUSCLE ALPHA-ACTIN;

D O I：

10.1016/0014-5793(91)80056-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of smooth muscle (sm) alpha-actin was studied in cloned capillary cerebral endothelial cells of two phenotypes. Type I cells were cultured in medium containing 10% FCS, heparin and ECGS (or alpha-ECGF) and stained positive for a specific endothelial cell marker (Bandeiraea simplicifolia). Depletion of heparin and ECGS resulted in a smooth muscle-like appearance after 2-3 days. Cells of this phenotype, (type II) stained positive for the endothelial cell marker and for sm alpha-actin. In contrast to type I cells, type II cells expressed sm alpha-actin protein and mRNA as evidenced by Immunoblots and Northern blots. This phenotypic switch was shown to be reversible and so was the expression of sm alpha-actin.

引用

页码：223 / 225

页数：3

共 24 条

[1] GAMMA-GLUTAMYL-TRANSPEPTIDASE (GGTP) AND NA+K+-ATPASE ACTIVITIES IN DIFFERENT SUBPOPULATIONS OF CLONED CEREBRAL ENDOTHELIAL-CELLS - RESPONSES TO GLIAL STIMULATION [J].