IDENTIFICATION OF RAT URINARY KININ AS BRADYKININ

被引：11

作者：

HAGIWARA, Y

KOJIMA, M

KURAISHI, T

HAYASHI, I

MIYATA, T

OHISHI, S

机构：

[1] KITASATO UNIV,SCH PHARMACEUT SCI,DEPT PHARMACOL,MINATO KU,TOKYO 108,JAPAN

[2] NATL CARDIOVASC CTR 1,RES INST,SUITA,OSAKA 565,JAPAN

来源：

LIFE SCIENCES | 1995年 / 57卷 / 10期

关键词：

BRADYKININ; URINE; URETER URINE;

D O I：

10.1016/0024-3205(95)02035-H

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Bradykinin (BK)-like activity, which was detected by BK-enzyme-immunoassay, was purified from 80 ml of ureter urine of Sprague-Dawley rats by Sephadex G 25 chromatography, FPLC, and reversed phase HPLC. The purified kinin fraction showed the same retention time as authentic BK on HPLC and produced contraction of isolated rat uterus, the contraction being suppressed by a B2-antagonist Hoe140. There was no other kinin detected on the HPLC at the corresponding retention time to kallidin, arginyl-BK or T-kinin. The peptide showed an amino acid sequence identical to that of BK by amino acid sequence analysis.

引用

页码：997 / 1002

页数：6

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FURUTOKATO S, 1985, J BIOL CHEM, V260, P2054

[3]

Girolami J P, 1986, Adv Exp Med Biol, V198 Pt A, P137

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