FLUORESCENT DYE PRIMER CYCLE SEQUENCING USING UNPURIFIED PCR PRODUCTS AS TEMPLATES - DEVELOPMENT OF A PROTOCOL AMENABLE TO HIGH-THROUGHPUT DNA-SEQUENCING

被引：14

作者：

TROWER, MK

BURT, D

PURVIS, IJ

DYKES, CW

CHRISTODOULOU, C

机构：

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 12期

关键词：

D O I：

10.1093/nar/23.12.2348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：2348 / 2349

页数：2

共 13 条

[1] MAGNETIC BEAD PURIFICATION OF M13-DNA SEQUENCING TEMPLATES [J].

ALDERTON, RP ;

ECCLESTON, LM ;

HOWE, RP ;

READ, CA ;

REEVE, MA ;

BECK, S .

ANALYTICAL BIOCHEMISTRY, 1992, 201 (01) :166-169

[2] PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH-FIDELITY AND HIGH-YIELD FROM LAMBDA-BACTERIOPHAGE TEMPLATES [J].

BARNES, WM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2216-2220

[3]

CHISSOE S L, 1991, Methods (Orlando), V3, P55, DOI 10.1016/S1046-2023(05)80164-1

[4] DIRECT SEQUENCING OF DOUBLE-STRANDED PCR PRODUCTS WITHOUT INTERMEDIATE FRAGMENT PURIFICATION - DIGESTION WITH MUNG BEAN NUCLEASE [J].

DOWTON, M ;

AUSTIN, AD .

NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3599-3600

[5]

FULTON LL, 1994, BIOTECHNIQUES, V17, P298

[6]

HANKE M, 1994, BIOTECHNIQUES, V17, P858

[7] DIRECT SEQUENCING OF PCR PRODUCTS IN AGAROSE-GEL SLICES [J].

KHORANA, S ;

GAGEL, RF ;

COTE, GJ .

NUCLEIC ACIDS RESEARCH, 1994, 22 (16) :3425-3426

[8] HIGH-THROUGHPUT DETERGENT EXTRACTION OF M13 SUBCLONES FOR FLUORESCENT DNA-SEQUENCING [J].

MARDIS, ER .

NUCLEIC ACIDS RESEARCH, 1994, 22 (11) :2173-2175

[9]

MUZNY DM, 1994, AUTOMATED DNA SEQUEN, P182

[10] LARGE-SCALE PRODUCTION OF DNA SEQUENCING TEMPLATES BY MICROTITRE FORMAT PCR [J].

ROSENTHAL, A ;

COUTELLE, O ;

CRAXTON, M .

NUCLEIC ACIDS RESEARCH, 1993, 21 (01) :173-174

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