TOWARD THE ELUCIDATION OF THE MECHANISM OF ATTACHMENT AND ENTRY OF MALARIA SPOROZOITES INTO CELLS - SYNTHETIC POLYPEPTIDES FROM THE CIRCUMSPOROZOITE PROTEIN OF PLASMODIUM-FALCIPARUM BIND CA2+ AND INTERACT WITH MODEL PHOSPHOLIPID-MEMBRANES

被引:6
作者
VERDINI, AS
CHIAPPINELLI, L
ZANOBI, A
机构
[1] SCLAVO SPA,ROME,ITALY
[2] ENIRIC SPA,I-00015 MONTEROTONDO,ITALY
关键词
D O I
10.1002/bip.360310602
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Through the joint use of CD, Fourier transform ir (FTIR), and attenuated total reflectance FTIR we have found that synthetic polypeptide models of the Plasmodium falciparum circumsporozoite (CS) protein repeat domain bind calcium ions in helicogenic environments. Ca2+-(NANP)n complexes (n greater-than-or-equal-to 20) interact vectorially with model phospholipid membranes orienting their polypeptide axes preferentially along those of the lipid acyl chains. It is proposed that the P. falciparum CS protein central region, rather than acting as a molecular lure helping the parasite to evade host immune control, plays, as a specific Ca2+ macroligand, a critical functional role during attachment, invasion, and development of the malaria parasite in the hepatic cell.
引用
收藏
页码:587 / 594
页数:8
相关论文
共 23 条
[1]   SYNTHETIC PEPTIDES FROM THE CIRCUMSPOROZOITE PROTEINS OF PLASMODIUM-FALCIPARUM AND PLASMODIUM-KNOWLESI RECOGNIZE THE HUMAN HEPATOMA-CELL LINE HEPG2-A16 INVITRO [J].
ALEY, SB ;
BATES, MD ;
TAM, JP ;
HOLLINGDALE, MR .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (06) :1915-1922
[2]   CONFORMATION AND IMMUNOGENICITY OF ENGINEERED REPEATING SEGMENT OF THE CIRCUMSPOROZOITE SURFACE PROTEIN OF PLASMODIUM-FALCIPARUM [J].
ARMAH, GE ;
NISHIKAWA, S ;
MIKI, S ;
OMATA, Y ;
NAKABAYASHI, T ;
TOMITA, K .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 38 (01) :135-140
[3]  
BERNARDI A, 1986, Patent No. 22560
[4]   ATTENUATED TOTAL REFLECTANCE FOURIER-TRANSFORM INFRARED STUDIES OF THE INTERACTION OF MELITTIN, 2-FRAGMENTS OF MELITTIN, AND DELTA-HEMOLYSIN WITH PHOSPHATIDYLCHOLINES [J].
BRAUNER, JW ;
MENDELSOHN, R ;
PRENDERGAST, FG .
BIOCHEMISTRY, 1987, 26 (25) :8151-8158
[5]   EXAMINATION OF THE SECONDARY STRUCTURE OF PROTEINS BY DECONVOLVED FTIR SPECTRA [J].
BYLER, DM ;
SUSI, H .
BIOPOLYMERS, 1986, 25 (03) :469-487
[6]   SYSTEMATICS IN THE INTERACTION OF METAL-IONS WITH THE MAIN-CHAIN CARBONYL GROUP IN PROTEIN STRUCTURES [J].
CHAKRABARTI, P .
BIOCHEMISTRY, 1990, 29 (03) :651-658
[7]   H-1-NMR STUDIES OF SYNTHETIC POLYPEPTIDE MODELS OF PLASMODIUM-FALCIPARUM CIRCUMSPOROZOITE PROTEIN TANDEMLY REPEATED SEQUENCE [J].
ESPOSITO, G ;
PESSI, A ;
VERDINI, AS .
BIOPOLYMERS, 1989, 28 (01) :225-246
[9]   METHOD FOR MEASURING BINDING OF SMALL MOLECULES TO PROTEINS FROM BINDING-INDUCED ALTERATIONS OF PHYSICAL-CHEMICAL PROPERTIES [J].
HALFMAN, CJ ;
NISHIDA, T .
BIOCHEMISTRY, 1972, 11 (18) :3493-&
[10]  
Hollingdale M. R., 1988, The Liver: Biology and Pathobiology., P1195