INTERACTION OF PROTEIN SRP19 WITH SIGNAL RECOGNITION PARTICLE RNA LACKING INDIVIDUAL RNA-HELICES

被引：48

作者：

ZWIEB, C

机构：

[1] Department of Molecular Biology, University of Texas Health Center at Tyler, Tyler, TX 75710

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 11期

关键词：

D O I：

10.1093/nar/19.11.2955

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Derivatives of human SRP-RNA were constructed by site-directed mutagenesis and tested for their ability to interact with protein SRP19. An RNA missing helix 6 barely interacts with SRP19, while the helix 8-deletion mutant retains much binding capability. A mutant RNA consisting just of helix 6 also binds the protein, but not as well as the unaltered molecule. SRP19 interacts to a full extent with the fourth mutant RNA composed of helices 6, 7, 8 and a portion of helix 5. It is concluded that helix 6-and not helix 8-is the major SRP19 binding site. Helices 7, 8 and portions of helix 5 contribute to the formation of a functional site. These results agree with data suggesting a proximity of helix 6 and the conserved part of SRP-RNA.

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页码：2955 / 2960

页数：6

相关论文

共 18 条

[1] SOLUTION STRUCTURE OF AN UNUSUALLY STABLE RNA HAIRPIN, 5'GGAC(UUCG)GUCC [J].

CHEONG, CJ ;

VARANI, G ;

TINOCO, I .

NATURE, 1990, 346 (6285) :680-682

[2] THE ORGANIZATION OF THE 7SL RNA IN THE SIGNAL RECOGNITION PARTICLE [J].

GUNDELFINGER, ED ;

KRAUSE, E ;

MELLI, M ;

DOBBERSTEIN, B .

NUCLEIC ACIDS RESEARCH, 1983, 11 (21) :7363-7374

[3] SRP-RNA SEQUENCE ALIGNMENT AND SECONDARY STRUCTURE [J].

LARSEN, N ;

ZWIEB, C .

NUCLEIC ACIDS RESEARCH, 1991, 19 (02) :209-215

[4] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE ENCODING THE 19-KDA PROTEIN OF SIGNAL RECOGNITION PARTICLE (SRP) - EXPRESSION AND BINDING TO 7SL RNA [J].

LINGELBACH, K ;

ZWIEB, C ;

WEBB, JR ;

MARSHALLSAY, C ;

HOBEN, PJ ;

WALTER, P ;

DOBBERSTEIN, B .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9431-9442

[5]

MANIATIS T, 1982, MOL CLONING

[6] A GENERAL-METHOD OF SITE-SPECIFIC MUTAGENESIS USING A MODIFICATION OF THE THERMUS-AQUATICUS POLYMERASE CHAIN-REACTION [J].

NELSON, RM ;

LONG, GL .

ANALYTICAL BIOCHEMISTRY, 1989, 180 (01) :147-151

[7] PROTEIN TRANSLOCATION ACROSS WHEAT-GERM MICROSOMAL-MEMBRANES REQUIRES AN SRP-LIKE COMPONENT [J].

PREHN, S ;

WIEDMANN, M ;

RAPOPORT, TA ;

ZWIEB, C .

EMBO JOURNAL, 1987, 6 (07) :2093-2097

[8] PROTEIN-TRANSPORT ACROSS THE ER MEMBRANE [J].

RAPOPORT, TA .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (09) :355-358

[9] DEFINING THE BINDING-SITE OF XENOPUS TRANSCRIPTION FACTOR-IIIA ON 5S RNA USING TRUNCATED AND CHIMERIC 5S RNA MOLECULES [J].

ROMANIUK, PJ ;

DESTEVENSON, IL ;

WONG, HHA .

NUCLEIC ACIDS RESEARCH, 1987, 15 (06) :2737-2755

[10] THE 54-KD PROTEIN OF SIGNAL RECOGNITION PARTICLE CONTAINS A METHIONINE-RICH RNA-BINDING DOMAIN [J].

ROMISCH, K ;

WEBB, J ;

LINGELBACH, K ;

GAUSEPOHL, H ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1990, 111 (05) :1793-1802